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Accueil du site > Séminaires > Séminaires 2022 > MSC Seminar. April 4th 2022. Olivier Thoumine (IINS, Bordeaux) : "Biophysical approaches to investigate the diffusional trapping of adhesion molecules at neuronal synapses"..

MSC Seminar. April 4th 2022. Olivier Thoumine (IINS, Bordeaux) : "Biophysical approaches to investigate the diffusional trapping of adhesion molecules at neuronal synapses".

Sauf mention contraire, les séminaires et les soutenances se déroulent à 11h30 en salle 454A du bâtiment Condorcet.


There will be a live diffusion with Zoom.

Monday April 4th, 11h30

Contact Jean-Baptiste Manneville to attend the visio-seminar

Biophysical approaches to investigate the diffusional trapping of adhesion molecules at neuronal synapses.

Olivier Thoumine Équipe Molécules d’adhérence cellulaire dans l’assemblage synaptique IINS - UMR 5297 - CNRS - Université de Bordeaux

Abstract : Neuroligins form a family of cell adhesion molecules implicated in synapse development, but the mechanisms that retain these proteins at synapses are still incompletely understood. In this talk, I will present a series of recent methodological developments from my group to tackle this issue. I will first show that surface-associated neuroligin-1 is diffusionally trapped at synapses, where it interacts with scaffolding elements of the post-synaptic density (Chamma et al., Nat Commun 2016). I will also present a newly released computer program, called FluoSim, which is an interactive simulator of membrane protein dynamics for live-cell and super-resolution imaging techniques (Lagardère et al. Sci Rep 2020). FluoSim was recently used to quantitatively interpret the dynamic behavior of neuroligin-1 over both short and long time ranges, and provide an estimate of neuroligin-1 copy numbers in synapses at steady-state (Lagardère et al. Front Syn Neurosci 2022). I will also present an assay combining the immobilization of neuroligin binders on micropatterned substrates with live-cell imaging of fluorescently-tagged neuroligin-1 in heterologous cells, to measure the kinetics of synaptic ligand-receptor interactions in a membrane environment (Piette et al., in preparation).

Figure legend Individual trajectories of membrane receptors simulated with FluoSim. The diffusion coefficient is color coded (blue = low diffusion, red = high diffusion), showing the diffusional trapping of neuroligin-1 membrane molecules at a synapse.


Contact : Équipe séminaires / Seminar team - Published on / Publié le 16 mars


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