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Home page > Séminaires > Séminaires 2022 > MSC Internal Seminar. September 5th 2022. 11:30. Irène Nagle (MSC) : "Magnetic muscular multicellular aggregates : focusing on fluid-like properties of tissue models and driving macroscopic organization".

MSC Internal Seminar. September 5th 2022. 11:30. Irène Nagle (MSC) : "Magnetic muscular multicellular aggregates : focusing on fluid-like properties of tissue models and driving macroscopic organization"

Sauf mention contraire, les séminaires et les soutenances se déroulent à 11h30 en salle 454A du bâtiment Condorcet.


Magnetic muscular multicellular aggregates : focusing on fluid-like properties of tissue models and driving macroscopic organization

Irène Nagle Equipe Physique du vivant

Abstract : Mechanics of biological tissues drives growing interest in mechanobiology to understand various processes such as morphogenesis, embryogenesis and tumorigenesis. It implies to develop new approaches to monitor and deform them. We address these challenges in the context of muscle tissue models by using magnetic muscle cells via the incorporation of biocompatible superparamagnetic nanoparticles (γ-Fe2O3). Magnetic labelling enables both the manipulation of cells at distance to create purely cellular aggregates of controlled shapes and the application of forces to measure their mechanical properties. Using mouse muscle precursor cells C2C12, we obtained multicellular aggregates without any support matrix of unprecedented size (>1 mm). The aggregate deformations under the application of a magnetic field enable to measure its macroscopic mechanical properties (surface tension, Young’s modulus). We looked at the interplay between the individual cell properties (cell-cell adhesions, actin structure and tension) and the mechanical properties at the tissue scale revealing the importance of desmin disorganization in macroscopic rigidity and surface tension. By studying desmin-mutated muscle precursor cells (point mutations involved in desminopathies), we enhanced the fundamental role of the intermediate filament network architecture in 3D tissue models. Moreover magnetic forces can be used to drive muscle cell differentiation by first reproducing their alignment and secondly stimulating them. We develop a magnetic stretcher to stretch multicellular aggregates of muscle precursor cells trapped between two mobile magnets and induce their differentiation into aligned muscular cells. The magnetic stretcher represents a new tool to explore cell deformation and muscle cell differentiation under mechanical stretching.


Contact : Équipe séminaires / Seminar team - Published on / Publié le 30 août


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