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Accueil du site > Séminaires > Archives séminaires > Séminaires 2014 > Séminaire interne MSC du Lundi 28 Avril 2014 : Elisabeth Charrier et Sham Tlili.

Séminaire interne MSC du Lundi 28 Avril 2014 : Elisabeth Charrier et Sham Tlili

Sauf mention contraire, les séminaires et les soutenances se déroulent à 11h30 en salle 454A du bâtiment Condorcet.

Séminaire interne du Lundi 28 Avril 2014

Elisabeth Charrier et Sham Tlili

Elisabeth Charrier

"Desmine, mécanique et myopathies myofibrillaires"

PNG - 232.7 ko

The cytoskeleton plays a central role in transmitting and generating mechanical forces through the cell. It is composed of three interconnected networks, actin, microtubules and intermediate filaments (IF). Desmin belongs to the type III IF, specifically expressed in muscles. Desmin is essential to maintain the integrity and functioning of muscles. More than fifty mutations have been identified in the gene encoding desmin leading to rare diseases belonging to MyoFibrillar Myopathy group (MFM). These pathologies are mainly characterized by aggregates formation in muscle tissue, associated with misorganizations of the contractile apparatus. Moreover patients progressively develop muscle weakness. Currently, pathophysiology and molecular defects of MFMs remain largely unknown, and no treatments are available.

In this context, the aim of our study is to clarify whether desmin mutations implicated in MFMs plays a role at early stage of expression, by impairing the properties of pre-muscular cells, the myoblasts. First we have studied the formation of desmin aggregates in living myoblasts over-expressing for 24h wild-type (WT) or different mutant desmins. We show that each mutant has a specific impact on the desmin network organization. Second we have performed mechanical measurements on C2C12 cells, focusing on the E413K mutant, which induces a large desmin network disorganization associated with important aggregate formation : we have compared the mechanical properties of WT-cells, C2C12 over-expressing desmin-WT-GFP and C2C12 overexpressing mutated desmin E413K-GFP. Visco-elastic properties of cells have been evaluated at the cortical and the whole-cells scale by using two custom-made set-ups, optical tweezers and a single-cell rheometer : we show that the 3 cells types share the same visco-elastic behaviour. Finally, we have investigated the impact of mutated desmin on the contractility of myoblasts, and we demonstrate that E413K-mutation significantly decreases cell contraction abilities specifically for cells with desmin aggregates, while aggregates of WT-desmin do no induce the same effect.

Sham Tlili

To be announced

Contact : Équipe séminaires / Seminar team - Published on / Publié le 23 avril 2014

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