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Magneto-fluorescent Nanowires for in Vitro Applications

MSC : Jean-François Berret (CNRS)

MSC : Leticia Vitorazi (Post-Doc)

Project description

Magnetic nanowires have attracted much interest recently because of their potential applications in sensing, micromechanics, microfluidics and cell manipulation. Magnetic nanowires are elongated objects in the µ-meter range, which can be actuated by the application of an external magnetic field.

Their motion of rotation provides information of the viscosity of the fluid. At the Laboratory Matière et Systèmes Complexes in Paris, we have developed a method to synthesize magnetic nanowires of length 1 – 10 µm that can be internalized into living cells and tissues. With these probes, we study the biomechanics of cells. To improve the contrast of the wires, we wish to add a fluorescent functionality while keeping the magnetic properties unchanged. Bimodal nanowires can be obtained by incorporating fluorescent semi-conductor nanoparticles, called quantum dots into the wires. The fluorescence properties of the quantum dots are modulated by a change of the particle size.

The objectives of the internship are :
-  The coating and characterization of quantum dots in solutions
-  The synthesis of bimodal wires with fluorescent and magnetic properties
-  The imaging of wires inside living cells at different stages of the internalization.

During the internship, the student (M1, M2) will have the opportunity to learn different techniques of physical-chemistry and biophysics, including the purification of nano/bio materials, spectroscopy, gel electrophoresis, optical and confocal microscopy and cell culture.

Description of the team that the student will join for the project

Describe the team that the student will join for the project. The intern will join a group of 6 researchers, composed of 1 PhD (Fanny Mousseau), 2 postdocs (Leticia Vitorazi, one to be hired) and 3 permanent positions. The permanent positions are an ANR awarded “Chaire d’excellence” (Emek Seyrek), a biology engineer (Carine Vias) and a CNRS scientist (Jean-François Berret). Our research group develops novel functional nanostructures with stimuli-responsive features and biocompatibility. The particles, proteins and other biomacromolecules are elementary bricks of colloidal scaffolds designed for applications. Based on techniques of assembly using non-covalent interactions, this approach offers versatility and simplicity for the fabrication of novel nanomaterials with enhanced functionalities. A second objective of our research deals with the applications of these nanomaterials in medicine, biology and in environment. It includes their use as tools for imaging and therapy in living cells and tissues, as well as the study of their cyto- and genotoxicity. In this research, emphasis is put on key features such as interactions, localization and titration of nanomaterials in biological and natural environments.

Recent References on this work

M. Safi, M. Yan, M.-A. Guedeau-Boudeville, H. Conjeaud, V. Garnier-Thibaud, N. Boggetto, A. Baeza-Squiban, Florence Niedergang, D. Averbeck and J.-F. Berret Interactions between magnetic nanowires and living cells : Uptake, toxicity and degradation, ACS Nano 5 (7), 5354-5364 (2011)

M. Safi, J. Courtois, M. Seigneuret, H. Conjeaud and J.-F. Berret, The effects of aggregation and protein corona on the cellular internalization of iron oxide nanoparticles, Biomaterials 32 (2011) 9353-9363

J.-P. Chapel and J.-F. Berret, Versatile Electrostatic Assembly of Nanoparticles & Polyelectrolytes : Coating, Clustering and Layer-by-Layer Processes, Current Opinion in Colloid and Interface Science 17, 97-105 (2012)

Nom des membres actuels ou anciens

BERRET Jean-François

Mot-clé général



Si les informations de cette page sont erronées ou incomplètes (par exemple la liste des thèmes de recherche), merci de contacter l'équipe web (lab-msc-web AT