May 19-23, 2014, Université Paris 7 Diderot
This is an informal workshop open to anyone who is actively performing, or who has performed, force or stress measurements in situ in living tissues.
Please note that participants are responsible for finding their own accommodation in Paris, and that we are unable to guarantee any funding.
Scroll down for the registration form (deadline February 28th, 2014), and for details of how to get there.
To facilitate discussions during the workshop, we would like you to submit an A4 2 page-document by May 12th (please see details below).
We start at 9:30 the first day.
We change slots of some methods. Please check the program below.
Understanding the interplay between genetics and mechanics in living tissues, especially during morphogenesis, is an active field of research. After years of emphasis on description of tissue deformation, cell-level dynamics, and molecular activities, the community currently devotes a large effort to measuring forces and stresses in situ. Several methods have recently been published, others are in preparation. This workshop aims at encouraging:
- discussions between researchers in the field
- exchange of published and unpublished information
- discussion of respective advantages and drawbacks of each method for any given tissue/organism
- mutual validations of independent methods
- link between different scales: molecule, cell, tissue
- improvement of existing methods
- development of new methods
- new collaborations
The workshop is purposedly informal:
- free registration (and no support for finding money or lodging)
- discussions and chalk-talks, rather than video-projections and invited speakers
At each discussion, participants will design a time-keeper and a secretary to take notes.
Discussions will be held approximately from 9h to 12h and from 14h30 to 17h30, with breaks (on monday, we start at 9h30). The exact program will be adapted according to the participants presence. Some time will be devoted to general discussions. Each method and its variants will be specifically discussed during one morning or afternoon.
- May 19. AM - Introduction
Scientific objectives : Kaoru Sugimura
Practical details : François Graner
Quick self-presentation by participants : background, field of interest
- May 19. PM - Tweezers
Magnetic tweezers : Ulrike Schulze
Optical tweezers : Raphael Clément
- May 20. AM - FRET
FRET measurements : Nicolas Borghi
Simulations : Simon Cox
- May 20. PM - Deformable sensors
Liquid drops : Otger Campas
Gel drops : Sham Tlili
Modelling aggregates : Françoise Brochard
- May 21. AM - Birefringence
Birefringence in animal tissues : Christof Aegerter
Birefringence in plant tissues : Evelyne Kolb
- May 21. PM - Micro-manipulation
Nano-indentation, atomic force microscopy : Léna Beauzamy
Pipette aspiration : Jean-Léon Maître
- May 22. AM - Laser ablation
Cell wall laser ablation : Loïc Le Goff
Tissue ablation : François Graner
- May 22. PM - Force inference
Static force inference : Shuji Ishihara and Kaoru Sugimura
Dynamic force inference : Wayne Brodland
- May 23. AM
Shapes and deformations at cell and tissue scale : Boris Guirao
General Discussion : Cross-validations between methods, Fields of applications
- May 23. PM
Linking models and experiments : Sascha Hilgenfeldt
General Discussion : Needs of the community, Dissemination of methods and results
The workshop is opened to anybody, from beginners to advanced users, who has been or is actively performing force or stress measurements in situ in living tissues, or modelling involved in corresponding methods.
To favor discussions, the number of participants is strictly limited. Registration, although free, is thus mandatory: scroll down for the registration form.
The selection of participants aims at balancing the different measurement methods, and also the fields of research.
A few seats might be kept for researchers in related fields such as:
- in vitro measurements of force and stress
- in vivo measurement of elastic and viscous moduli
Christof Aegerter
Wylie Ahmed
Adrien Baldit
Abdul Barakat
Léna Beauzamy
Nicolas Borghi
Françoise Brochard-Wyart
G. Wayne Brodland
Otger Campas
Clément Campillo
Raphaël Clément
Simon Cox
Hélène Delanoë-Ayari
Julien Dumortier
Charlène Gayrard
François Graner
Karine Guevorkian
Boris Guirao
Adrien Hallou
Takashi Hiiragi
Sascha Hilgenfeldt
Shuji Ishihara
Evelyne Kolb
Yohei Kondo
Pascal Kurowski
Flavio Lanfranconi
Loic Le Goff
Pierre-François Lenne
Jean-Léon Maître
Philippe Marcq
Vincent Nier
Eric Raspaud
Ulrike Schulze
Kaoru Sugimura
Sham Tlili
Hervé Turlier
To facilitate discussions during the workshop, we would like you to submit an A4 2 page-document by May 12th. A booklet will be provided via e-mail before the workshop.
- Free style, PDF format - Contain the following informations:
1. Title:
2. Name:
3. Lab and Institution:
4. Introducing a method: the principle of the method, the set-up; the systems on which it can work; the advantages and drawbacks; the range of size, of forces, or price involved; etc.
5. Research results:
6. Motivation to attend the workshop: Discussions to focus ideas; Possibility of cross-validation; etc.
- Send to tissue.stress@univ-paris-diderot.fr
It will take place on May 19 to 23, 2014 in Univ. Paris 7 “Denis Diderot”, Paris Rive Gauche campus, Condorcet A building, 3rd floor. We meet in room 366A-Klimt. (Note : A smaller room is available for parallel discussions or private work)
Please read carefully the following explanations and print the maps.
The “Paris Rive Gauche” campus of University Paris 7 "Denis Diderot" is in the south-east of Paris (XIIIth district), between the National Library and the ringroad. It can be accessed from the station “Bibliothèque François-Mitterrand” of RER C or subway line 14, by buses 89, 62, 64, 325, and by the “velib” bicycles.
The Condorcet building is close to the Seine river and has a distinct orange color. From subway line 14, RER C or bus 89 (arrêt “Bibliothèque François Mitterrand”) go to the Seine by walking downhill, then turn right. See the map of the district (move your mouse to see the Condorcet building on the bottom left) and the detailed map of the building. On these maps, green dots label the building entrances.
Caution: these streets are new. Some commercial maps or GPS systems have not yet incorporated them. Watt street, which is almost identical to Alice Domon and Léonie Duquet, is normally always indicated.
Caution again: this building is subdivided into two subunits, Condorcet A and Condorcet B, which are connected only at the ground floor (as well as a footbridge at 4th floor and another one at 6th floor).
Caution again: at night it is only possible to leave this building through the guarded door on 10 rue Alice Domon and Léonie Duquet, close to the river.
Participants are responsible for finding their own accommodation in Paris, or south-east suburb such as Ivry.
Wifi for academic participants: eduroam is available at the venue, but you must register at your home institution in advance; if you need an alternative wifi, please let us know on the registration form.
Visa : Persons who need an acceptance letter for visa should contact us quickly.
Food : The food from monday, May 19th to thursday, May 22nd will be at University Restaurant Buffon. There will be a self-service starter buffet followed by a main dish: Monday : lamb, Tuesday : salmon, Wednesday : duck, Thursday : shrimps This will be paid by the workshop. If you want a modification of this menu (eg for dietary, allergy or personal reasons, including vegetarians), please notify us before may 5th at latest. If you don't want to eat there, you can go to nearby restaurants or bring your own food. In that case, please notify us before may 5th at latest. For Friday, May 23rd, we will organise a buffet to eat together in the lab. If you can bring something to eat, to share with others, it would be nice (for instance a specialty from your home town).
Coffee breaks : Coffee will be provided by the workshop : 3 coffees per person and per day. If you can bring biscuits or cakes, to share with others, it would be nice.
Organisers will apply for a limited funding if one or two participants need support.
Please send before February 28th, 2014 an e-mail in english to tissue.stress@univ-paris-diderot.fr with:
- Name and surname:
- Lab and institution:
- Field of research (eg biophysics, developmental biology, mechanics, optics, …):
- Method(s) which you have used, use or will use:
- Tissue(s) and model organism(s) on which you work:
- If you are not exactly in the field, explain your motivations to attend:
- The days you plan to attend, if you already know them:
- Any comments, or information useful for the organisers:
tissue.stress@univ-paris-diderot.fr
http://www.msc.univ-paris-diderot.fr/tissue-stress/
Organisers :
Kaoru Sugimura, Kyoto, Japan
Pierre-François Lenne, Marseille, France
François Graner, Paris, France
