Séminaires MSC
"Matière et Systèmes Complexes"


Lundi 14 mai 2007 à 11h30
Bâtiment Condorcet, 4ème étage, salle 454 A.

Miglena Angelova
INSERM U538, CHU Saint-Antoine)

Heterogeneous Membranes and Some Physical Chemistry of Membrane Interactions. The Role of Lipid « Rafts

Vesicles are closed membranes of spherical shape separating a water compartment from the bulk water in which the vesicle is suspended. 
Cell-sized or giant unilamellar vesicles (GUV), 10 to100 µm in diameter, are quite efficiently prepared by the liposome electroformation method.
Recently the method was used for preparation of GUVs containing raft-like domains visible under optical microscope.
The raft-like domain containing GUVs permeated studies of the role of membrane heterogeneity and compartimentalisation in the bilayer membrane interactions,
shape and topology transformations. Some questions regarding cell structure and functions evoked in our recent studies will be presented:
the controversial regarding biological “rafts” and the preparation of detergent resistant membranes (DRM), the phospholipase A2 activity,
the mechanisms of cholesterol extraction from membrane by the high density lipoproteins (HDL),
the morphology and the dynamics ofmitochondrial cristae. At the end, we shall present our forthcoming projects.