Séminaire MSC
"Matière et Systèmes Complexes"


Lundi 22 juin 2009 à 11h30
Bâtiment Condorcet, 4ème étage, salle 454 A.

Hélène Berthoumieux

In vivo characterization of enzymatic networks dynamics

Biologists, biophysicists and chemists are interested in the description of biochemical species and in the copy of their dynamical properties. For a good characterization of the kinetics of such objects, an in situ investigation is essential. During the last 10 years, biologists have developed efficient in vivo reporters and biophysicists use that detection tools for the determination of gene regulation dominant process. The long time scale phenomena are thus investigated. As chemists, we describe a biological medium as a set of chemical reaction networks. Their characterization implies the determination of their topology, the nature of the steady state and the values of the associated rate constants. In particular, we are interested in enzymatic catalysis. Using temperature modulation, we propose a protocol to collect information on the topology of such networks and we present a method for the determination of all the rate constants of a Michaelis-Menten mechanism, which makes a detailedbalance test possible.