Séminaire Exceptionnel
Mercredi 17 mai 2006 à 11h30
Tour 33, couloir 33-43, 2ème étage, salle de réunion

Hacene Boukari
(NIH, Bethesda)

Probing the dynamics of nanostructures in congested media by fluorescence correlation spectroscopy

Diffusion and transport of macromolecules through inhomogeneous media are important dynamical phenomena relevant to many biological activities
and physico-chemical processes. 
Examples are: intracellular protein trafficking, supramolecular assembly, virus penetration, protein separation, and drug delivery. 
We have exploited the advantages of fluorescence correlation  spectroscopy (FCS) to measure the diffusion and interactions of various
probes (~1-100 nm) in different concentrated polymeric solutions and synthetic biogels prepared under different conditions. 
In this talk, I will describe briefly the technique and focus on the results of analysis of these measurements, where we attempt to correlate changes in the dynamics of the probes with corresponding bulk viscoelastic properties of the host medium.