"Matière et Systèmes Complexes"
12 novembre 2007 à 11h30
Bâtiment Condorcet, 4ème étage, salle 454 A.
(Unitat de Biofisica i Bioenginyeria, Facultat de Medicina, Barcelona)
Cell mechanical properties play a key role in many important cell functions including contraction, migration, proliferation and differentiation. Moreover, these processes are mediated by the ability of the cell to feel and respond to mechanical stresses and to the mechanical features of the microenvironment. Yet the mechanisms underlying the mechanical properties of the cells and their interplay with the microenviroment remain largely unknown. To further our understanding of the physical laws governing cytoskeleton rheology and dynamics we study temperature and ATP dependences of the cell complex elastic modulus with atomic force microscopy and the spontaneous cytoskeleton fluctuations with particle tracking microscopy. To ascertain the cell response to the adhesive and mechanical features of the microenvironment we assess changes in cell mechanics and proliferation induced by cell-substrate adhesion by using atomic force microcopy and microcontact printing. We also address the rheological and contractile response of the cell to stretching by means of magnetic tweezers and traction microscopy.