Séminaires MSC
"Matière et Systèmes Complexes"

Lundi 9 janvier 2006 à 11h30
Tour 33, couloir 33-43, 2ème étage, salle de réunion

Manuel Théry
(Institut Curie, CNRS UMR144, Paris)

Adhesive control of cell polarity and bipolarity

Cells in tissus receive spatial informations from the spatial distributions of the contacts they have with other cells or with the extra-cellular matrix around them. In order to analyse and quantify the role of this distribution of adhesion onto the cellular organisation (cytoskeleton and compartimentalisation) we made various micropatterns imposing cells to have similar shapes but distinct adhesive distributions. We showed that actin dynamics at the cell cortex respond to the localisation of adhesive and non adhesive zones regardless of cell shape. Cell cortex was polarised in contraction zones upon non-adhesive edges and membranes ruffles upon adhesive one. This peripheral polarity was then transmitted to the internal polarity in interphase and to the bipolar spindle orientation during mitosis.